Factors Affecting Enzyme Activity Flashcards

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What are the four factors that affect enzyme activity?

Temperature, pH, enzyme concentration and substrate concentration.

As temperature increases, enzyme activity increases until an optimum point. Beyond this, high temperatures denature the enzyme.

Approximately 37°C.

Most enzymes work best at pH 7. Extreme pH levels can denature enzymes by altering the shape of the active site.

The rate of reaction increases until the active sites become fully occupied, after which it remains constant.

The rate of reaction increases with substrate concentration until all the enzyme active sites are occupied, then it remains constant.