Factors Affecting Enzyme Activity Flashcards

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What are the four factors that affect enzyme activity?

Temperature, pH, enzyme concentration and substrate concentration.

How does temperature affect enzyme activity?

As temperature increases, enzyme activity increases until an optimum point. Beyond this, high temperatures denature the enzyme.

What is the optimum body temperature for human enzymes?

Approximately 37°C.

How does pH affect enzyme activity?

Most enzymes work best at pH 7. Extreme pH levels can denature enzymes by altering the shape of the active site.

What happens to enzyme activity as enzyme concentration increases?

The rate of reaction increases until the active sites become fully occupied, after which it remains constant.

How does substrate concentration affect enzyme activity?

The rate of reaction increases with substrate concentration until all the enzyme active sites are occupied, then it remains constant.

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