Monoclonal Antibodies Flashcards

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What are monoclonal antibodies?

Monoclonal antibodies are identical copies of a single type of antibody produced from clones of a unique white blood cell.

How are antibodies produced in the body?

Antibodies are produced by lymphocytes, a type of white blood cell, in response to foreign antigens on pathogens.

What are antigens and how do antibodies interact with them?

Antigens are proteins on the surfaces of pathogens. Antibodies are complementary to specific antigens and bind to them, immobilising the pathogens.

What is the first step in producing monoclonal antibodies?

Injecting a mouse with an antigen to stimulate lymphocytes to produce antibodies in response.

How are hybridoma cells formed?

Hybridoma cells are formed by fusing lymphocytes with tumour cells (myeloma cells), which can divide by mitosis and produce antibodies.

How are monoclonal antibodies collected and purified?

Hybridoma cells are allowed to divide, forming identical clones that produce identical antibodies, which are then collected and purified in large amounts.

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