The Process of Genetic Engineering Flashcards

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What is the first step in the process of genetic engineering?

The desired characteristic is selected, and the gene responsible for this characteristic is located in the original organism.

What role do restriction enzymes play in genetic engineering?

Restriction enzymes are used to cut out the gene responsible for the desired characteristic, leaving ‘sticky ends’ with unpaired bases.

How are ‘sticky ends’ joined together in genetic engineering?

DNA ligase is an enzyme that can join ‘sticky ends’ if the nucleotides on the strands are complementary.

What is a vector in genetic engineering, and how is it used?

A vector, usually a bacterial plasmid or a virus, is used to insert the gene into the cells of the target organism.

Why must the gene be inserted during the early stages of development in genetic engineering?

The gene must be inserted during the early stages, like the embryo phase in animals, to ensure all cells receive the gene and the organism develops with the desired characteristics.

What is the final step in the process of genetic engineering?

The vector (bacterial plasmid or virus) is used to insert the gene into the cells of the target organism.

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