Cells and Microscopy Flashcards

1️⃣ Familiarise yourself with the flashcards:

  • Look through all the flashcards to see what's on both sides.
  • Make sure you understand the information on each card. If something's unclear, click the link to the revision notes at the bottom of the page for more details.

2️⃣ Test yourself:

  • Look at the question or prompt on each card and try to remember the answer before flipping it over.
  • Check the answer and make a note of any cards you find challenging and need to go over more.

3️⃣ Consistently Review and Practice:

  • Use spaced repetition: spend more time on the cards you struggle with and go over them more often.
  • Regularly review all the flashcards to help you better understand and retain the information over time.

Note: We may include questions that have multiple correct answers. It's useful to remember specific examples to understand these concepts better.

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What does a light microscope let you do?

See small structures in cells by making them look bigger.

How do you find total magnification on a light microscope?

Multiply the eyepiece magnification by the objective magnification.

What is the formula linking magnification, image size and real size?

Magnification = image size ÷ real size.

How are mm, μm and nm related?

1 mm = 1000 μm and 1 μm = 1000 nm.

What does resolution mean in microscopy?

How well you can see two close points as separate.

What is a wet mount slide?

A slide made by putting a specimen in a drop of liquid and covering it with a coverslip.

How do you make a wet mount of onion skin?

Put a drop of water on the slide, add a thin onion layer, add iodine solution, then lower the coverslip at an angle.

Why do you lower the coverslip at an angle?

To avoid trapping air bubbles.

Why start with the lowest power objective lens?

It gives a wider view and helps prevent damage to the slide.

How do you focus a light microscope safely?

Use coarse focus on low power to find the image, then use fine focus to sharpen it.

What happens to the field of view as magnification increases?

It gets smaller.

An image is 30 mm wide at ×300. What is the real width in μm?

30 mm ÷ 300 = 0.1 mm = 100 μm.