Enzymes Flashcards

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What is an enzyme?

An enzyme is a biological catalyst. It speeds up reactions without being used up.

What is the active site of an enzyme?

It is the part of the enzyme that the substrate binds to. It has a specific shape.

What does the lock and key model show about enzymes?

It shows that only the correct substrate fits the enzyme's active site, like a key in a lock.

What is a substrate?

The molecule that an enzyme acts on.

How does temperature affect enzyme activity up to the optimum?

As temperature rises, the rate increases. It reaches an optimum near 37°C for many human enzymes.

What happens to enzymes at high temperatures?

They denature. The active site changes shape so the substrate no longer fits.

How does pH affect enzymes?

Each enzyme has an optimum pH. Too high or too low pH can denature it.

What reaction does amylase catalyse?

Amylase breaks starch into sugars, such as maltose.

What reaction do proteases catalyse?

Proteases break proteins into amino acids.

What reaction do lipases catalyse?

Lipases break lipids (fats) into glycerol and fatty acids.

How does substrate concentration affect enzyme activity?

A higher substrate concentration increases the rate. It then levels off when all active sites are full.

How can you calculate the rate from the time taken for an enzyme reaction?

Rate = 1 / time. Use seconds for time.