Food Tests Flashcards

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  • Make sure you understand the information on each card. If something's unclear, click the link to the revision notes at the bottom of the page for more details.

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Note: We may include questions that have multiple correct answers. It's useful to remember specific examples to understand these concepts better.

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What do food tests check for?

They check for starch, reducing sugars, protein, and lipids in foods.

How do you prepare a food sample before testing?

Mash the food with distilled water. Filter to remove lumps, and use the liquid.

Which reagent tests for starch, and what is a positive result?

Iodine solution. It changes from orange-brown to blue-black if starch is present.

How do you test for reducing sugar?

Add Benedict’s solution to about 5 cm³ of the sample and heat in a 75 °C water bath for 5 minutes. A colour change from blue shows sugar is present.

What do the colour changes in Benedict’s test mean?

Blue = none. Green = a little. Yellow or orange = more. Brick-red = lots.

How do you test for protein?

Add Biuret reagent to the sample. It turns from blue to lilac or purple if protein is present; if not, it stays blue.

How do you test for lipids using Sudan III?

Add a few drops of Sudan III and shake gently. A red oil layer forms on top if lipid is present; if not, no separate red layer appears.

Why should you not filter the sample before the lipid test?

Filtering can remove the fat. The test may then miss lipids that are there.

Why use a water bath for Benedict’s test?

It heats the sample safely and evenly to about 75 °C without a flame.

How do you keep food tests fair when comparing foods?

Use the same volumes, the same temperature, and the same time for each test.