Aseptic Techniques Flashcards

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What does aseptic technique mean?

Working in a way that keeps equipment and cultures free from unwanted microbes.

Why is aseptic technique important when growing bacteria?

It prevents contamination, keeps you safe, and makes results reliable.

What should you do before starting work to reduce contamination?

Wash your hands, disinfect the bench, and tie back long hair.

How do you sterilise an inoculating loop before use?

Hold it in a Bunsen flame until it is red hot, then let it cool.

Why do you flame the neck of a culture bottle?

To kill microbes on it and create warm air that helps keep other microbes out.

How should you handle a Petri dish when inoculating?

Open the lid only a little, keep it over the dish, and work quickly.

Why do you work near a Bunsen burner during inoculation?

The flame makes an updraft that reduces airborne microbes settling on the plate.

Why are agar plates incubated at about 25°C in school labs?

It reduces growth of harmful human pathogens but still lets many bacteria grow.

Why do you tape, but not fully seal, the lid of a Petri dish?

Taping keeps the lid on and stops contamination, but gaps let oxygen in.

Why are inoculated plates stored upside down?

To stop condensation dripping onto the agar and spreading the microbes.

How are media and equipment sterilised before use?

Use an autoclave or pre-sterilised items to kill all microbes.

How should used cultures be disposed of safely?

Keep lids on and sterilise them, for example in an autoclave or with disinfectant.