Calculating Zone of Inhibition Area Flashcards

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In an antibiotic disc test, what is the zone of inhibition?

It is the clear area around the disc where bacteria have not grown.

Why do we calculate the area of the zone of inhibition?

To compare how well different antibiotics stop bacteria growing.

What shape do we assume a zone of inhibition is when we calculate its area?

We assume the zone of inhibition is a circle.

What is the formula for the area of a circular zone of inhibition?

Area = π × radius².

How do you find the radius if you know the diameter of a zone of inhibition?

Divide the diameter by 2 to get the radius.

When measuring the diameter of a zone of inhibition, where do you measure?

Measure from one edge of the clear zone, through the centre of the disc, to the opposite edge.

If the diameter of a zone is 18 mm, what is its radius?

The radius is 9 mm, because radius = diameter ÷ 2.

If the radius of a zone is 7 mm, how do you work out its area?

Use area = π × 7², so area ≈ 3.14 × 49 ≈ 154 mm².

What are the steps to calculate zone of inhibition area from its diameter?

Measure the diameter, divide by 2 to get the radius, then use area = π × radius².

If two zones have diameters of 14 mm and 28 mm, how do their areas compare?

The 28 mm zone has four times the area, because area uses radius squared.

What units do you use for zone of inhibition area if the diameter was measured in millimetres?

Use square millimetres, written as mm².

Why is area a better measure than just diameter for zone of inhibition size?

Because area shows the whole size of the clear region, not just its width.