Isolation and Reinfection in Plant Disease Flashcards

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In plant disease studies, what does isolation mean?

Isolation means taking a suspected pathogen away from the plant and other microbes so it is on its own.

Why do scientists isolate microorganisms from a diseased plant?

They isolate them to find out which microorganism might be causing the disease.

What is the first step before isolating a pathogen from a plant?

The first step is to look for and record the symptoms on the diseased plant.

What does reinfection mean in plant disease investigations?

Reinfection means putting the isolated microorganism onto a healthy plant to see if it causes disease.

Why do scientists reinfect healthy plants with an isolated microorganism?

They do this to test if that microorganism really causes the disease, not just lives on the plant.

What result shows that an isolated microorganism is the cause of a plant disease?

Healthy plants given the microorganism get the same symptoms as the original diseased plant.

Why do scientists use control plants in reinfection experiments?

Control plants are not given the microorganism, so they show what happens without the suspected pathogen.

Why should the same plant species be used for reinfection as in the original disease case?

Using the same species makes the test fair, so differences are due to the microorganism, not the plant type.

How does isolation and reinfection help show cause, not just correlation, in plant disease?

If only plants with the isolated microbe get sick, it suggests the microbe causes the disease, not just happens to be there.

Why might some microbes from a diseased plant not cause disease when used for reinfection?

They may be harmless microbes that normally live on plants or are just contaminants.

What should scientists do with plants after a reinfection experiment is finished?

They should safely destroy or contain infected plants to stop the disease spreading outside the experiment.

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