Zones of Inhibition in Antibiotic Tests Flashcards

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In an antibiotic disc test, what is a zone of inhibition?

It is the clear area around an antibiotic disc where bacteria do not grow.

What does a clear zone of inhibition show about the bacteria?

It shows that the antibiotic has killed the bacteria or stopped them growing there.

How does a zone of inhibition form around an antibiotic disc?

The antibiotic diffuses out into the jelly and kills or stops nearby bacteria, leaving a clear circle.

What does a larger zone of inhibition usually mean about an antibiotic?

A larger zone usually means the antibiotic is more effective against that type of bacteria.

What does it suggest if there is no zone of inhibition around an antibiotic disc?

It suggests the bacteria are not affected by that antibiotic, so they may be resistant.

Why do scientists measure the diameter of the zone of inhibition?

They measure the diameter to compare how effective different antibiotics are on the same bacteria.

What unit is usually used to measure the zone of inhibition diameter?

The diameter is usually measured in millimetres (mm).

How can you compare two antibiotics using their zones of inhibition on the same plate?

You compare the diameters of their clear zones; the one with the bigger zone is usually more effective.

Why should all antibiotic discs be placed on the same type of agar with the same bacteria when comparing zones of inhibition?

So that only the antibiotic changes, making the comparison between zones fair and accurate.

What does a small but clear zone of inhibition tell you about an antibiotic?

It tells you the antibiotic works, but is less effective than one that makes a larger clear zone.